TABLE 3.
Nramp1 genotype distribution among brucellosis casesa
| Genotype | Frequencies of seropositive animals
|
|
|---|---|---|
| Observed | Expected (formula for expected frequency) | |
| AA | 71 | 56 (n × p2) |
| AB | 24 | 23 (n × 2pq) |
| AC | 2 | 18 (n × 2pr) |
| AD | 10 | 27 (n × 2ps) |
| BB | 0 | 2 (n × q2) |
| BC | 2 | 3 (n × 2qr) |
| BD | 10 | 5 (n × 2qs) |
| CC | 3 | 1 (n × r2) |
| CD | 17 | 4 (n × 2rs) |
| DD | 3 | 3 (n × s2) |
| Total | 142 | 142 |
a
p, q, r, and s indicate the observed frequencies of the Nramp1A, -B, -C, and -D alleles, respectively. p = (AA + AB/2 + AC/2 + AD/2)/n = 0.63; q = (BB + AB/2 + BC/2 + BD/2)/n = 0.13; r = (CC + AC/2 + BC/2 + DC/2)/n = 0.10; s = (DD + AD/2 + BD/2 + CD/2)/n = 0.15. n (number of observed animals) = 142. Degrees of freedom (DF) were calculated according to the formula k(k-1)/2, where k is the number of alleles (39). χ2 = {∑[(observed frequency − expected frequency)2/expected frequency]} = 79.8; DF = 6; P < 0.001.