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SDS-PAGE-separated proteins and LOS from a whole-cell lysate (WCL) of strain 35E were immunoblotted with 5 μg/ml of 35E LOS-specific IgG. Binding occurred to LOS but not to proteins. The binding of 35E LOS IgG to purified 35E LOS is also shown to indicate the location of LOS molecules when they were electrophoresed on a minigel.
