TABLE 3.
Presence of tccP2 and Tir type among 13 strains of EHEC O103
| Serotypea | No. of strains | VT | Intimin type | tccP2 product (bp) | Tir typeb | Origin |
|---|---|---|---|---|---|---|
| O103:H2* | 3 | 1 | e | 750 | E2348/69-like | Japan |
| O103:H2* | 1 | 1 | ɛ | 900 | E2348/69-like | Japan |
| O103:H2*† | 1 | 1 + 2 | ɛ | 750 | E2348/69-like | Japan |
| O103:H2*‡ | 1 | 1 | ɛ | 750 | E2348/69-like | France |
| O103:H2 | 1 | 1 + 2 | ɛ | 900 | E2348/69-like | Argentina |
| O103:H2 | 1 | 1 | ɛ | 900 | NT | Argentina |
| O103:H2 | 2 | 1 | ɛ | E2348/69-like | Argentina | |
| O103 | 2 | 1 | ɛ | 900 | E2348/69-like | Italy |
| O103 | 1 | 2 | ɛ | ND | Italy |
a
*, tccP2 was sequenced; †, amino acid sequences and expression of TccP2 of a representative isolate (strain 12009) are shown in Fig. 1 and 2, respectively; ‡, production of TccP by this strain (strain PMK5) is shown in Fig. 2.
b
Tir typing was done by discriminatory PCR primers. ND, not done; NT, not typeable.