Skip to main content
. 2006 Dec 11;75(2):574–580. doi: 10.1128/IAI.00985-06

FIG. 2.

FIG. 2.

SseL is expressed inside macrophages in an SsrB-dependent manner. (A) GFP expression of intracellular bacteria in BMDM. BMDM were infected with wild-type (wt) SL1344 (blue) or ssrB::aphT mutants (green), each containing psseL-gfp, and harvested 30 min postinfection for flow cytometry. Fluorescence intensity of intracellular bacteria expressing GFP in F-480-positive macrophages compared with baseline fluorescence of uninfected cells (red) is represented. (B) Number of BMDM containing intracellular bacteria expressing GFP. BMDM were infected as described above and harvested 30 min (gray bars) and 8 h (black bars) postinfection for quantitative analysis of F-480/GFP-double-positive macrophages by flow cytometry. Error bars represent standard deviations of triplicate samples representative of experiments performed twice. SL1344 versus ssrB::aphT: 30 min, P = 0.0003; 8 h, P = 0.0015.