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Stx1 and Stx2 circulate in the plasma in vivo. Groups of eight mice were injected intravenously in the tail with either Stx1 (A) or Stx2 (B) and bled from the retro-orbital sinus at 5 and 30 min after injection. Cells were separated from the plasma by centrifugation, and Stx levels were determined by ELISA. Values refer to the means ± standard deviations for values at 5 and 30 min after injection.
