FIG. 1.
Antibody and cellular response of GTF-immunized rats to linear GTF sequence peptides. (A) Pooled rat sera from three immunized rats or from two control (ctl) rats were assessed for antibody binding to linear 20-mer peptides derived from S. sobrinus GTF-I sequence by ELISA. Data bars represent the ratio of the immunized level of IgG to the IgG level without immunization (optical density at 405 nm for each). Asterisks indicate ratio levels that were different from those for the other peptides when tested by one sample t test; statistically significant, P < 0.05. (B) Proliferation of lymph node cells of two GTF-immunized rats and one control rat 10 days after immunization with GTF. Cultured cells were stimulated with GTF or the respective peptide and [3H]thymidine (added for the last 18 h of culture). Data presented as bars are the average ratios of immunized proliferation indices/unimmunized proliferation indices. Asterisks indicate ratios that were higher than those for the other peptides when tested by one sample t test; statistically significant, P < 0.05.