FIG. 3.

Immune reactivity of MAP construct-immunized rats with GTF. Rats (five or six/group) were immunized with 50 μg MAP construct 7, 11, or 16 either s.c. in the salivary gland vicinity (sgv) with CFA (A, C, and D) or i.n. (IN) with cholera toxin (B). Serum IgG antibody to GTF (ELISA units [EU]), group means, and standard errors of the means are indicated by bars. Serum was harvested 3 weeks post-booster immunization. ctl, control. ***, levels higher than those of other groups by SNK multiple-comparisons test; statistically significant, P < 0.001. *, levels higher than those of other groups by SNK multiple comparison test; P < 0.05. (C) Saliva harvested 3 weeks post-s.c. booster immunization contained mean IgA (EU) levels to GTF, shown as bars and standard errors, after MAP 7, 11, or 16 immunization. **, SNK, multiple comparison test; P < 0.01. (D) Serum inhibited GTF-mediated incorporation of [¹⁴C]glucose from sucrose into water-insoluble glucan. Mean percent inhibition of GTF activity by serum is indicated by bars with standard errors. **, SNK multiple comparison test, statistically significant (P < 0.01).