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Analysis of lipooligosaccharides purified from NTHi 86-028NP inoculum and isolates from chinchilla middle ear fluids. LOS were separated by Tricine-SDS-PAGE and visualized by ammonium-silver stain (A) or immunoblot using anti-PCho monoclonal antibody (B). Lanes: 1, inoculum; 2, 6 days postinfection; 3, 8 days postinfection; 4, 10 days postinfection; 5, 14 days postinfection.
