TABLE 2.
Oligonucleotide primers
| Primer | Oligonucleotide sequencea |
|---|---|
| TDE0108FLIC | GACGACGACAAGATTACTTTCAAAATGAATACTGCAC |
| TDE0108RLIC | GAGGAGAAGCCCGGTTTACTTTATCTTTTTGGGTAT |
| TDE1135FLIC | GACGACGACAAGATCTGCACAAGAAGCGGAATA |
| TDE1135RLIC | GAGGAGAAGCCCGGTTCAATCTTCTTTTGCTTTTCC |
| TDE2448FLIC | GACGACGACAAGATTAAGAGCCGCCGAATCGCCGAAC |
| TDE2448RLIC | GAGGAGAAGCCCGGTTTATTTCTTTTCGATTTTGCG |
| TDE0429FLIC | GACGACGACAAGATTAAAACAACCGATACAAGTAAAA |
| TDE0429RLIC | GAGGAGAAGCCCGGTTTAGATAGGCTTCAATATAAGC |
| TDE1191FLIC | GACGACGACAAGATTTCTAAGACAGCGATAAAGGC |
| TDE1191RLIC | GAGGAGAAGCCCGGTTTAGTACTCTCCACTATTGAGC |
| TDE1190FLIC | GACGACGACAAGATTAAAACAAATGAGAAAAAAAATGCTC |
| TDE1191RLIC | GAGGAGAAGCCCGGTCTAATATTCCGTATGCTTAAAATC |
| TDE1361FLIC | GACGACGACAAGATTAAACAATTTATTGCCGATATTG |
| TDE1361RLIC | GAGGAGAAGCCCGGTTTAAGCTCGTAGTCGGTACCATTG |
| TDE0940FLIC | GACGACGACAAGATCAAGACAAAGCAAATTCAGCC |
| TDE0940RLIC | GAGGAGAAGCCCGGTCTATAATTCGATATTAAAAACATTC |
| FhbB RT F | ACGCGCTTGAGAATGAATTA |
| FhbB RT R | AATCTAATGCAAGGGCTTCAG |
| FlaA RT F | GCTCAGGTTGATGATCAGG |
| FlaA RT R | GCAATTGATTTGATAACGCCG |
| TDE0109R1 | GCTCATCAGCTTGCAAAGGC |
| TDE0109R2 | CGATATTCATGACGTTTACTAC |
| FhbB Up | CTCTTGACAGTACGTATAGTG |
| FhbB78R | GGGTTTTTTATCCACAATTTG |
a
Underlining indicates the tail sequences added to allow annealing into the pET32 Ek/LIC vector.