FIG. 3.

Following inhalation, F. tularensis LVS expressing GFP colocalized with proSP-B and proSP-C, proteins produced by ATII epithelial cells. Bacterial localization was determined with a fluorescently labeled antibody to GFP (green). Nuclei were stained with DAPI (blue). Sections were probed with fluorescently labeled antibody to proSP-B (red) (A and B), proSP-C (red) (C and D) to identify ATII cells and β-tubulin (red) (E). Representative images are from lung sections 3 days postinoculation.