TABLE 1.
Macrophage groupa | Treatment | Mean no. ± SDb
|
||
---|---|---|---|---|
Infected macrophagesc | Total parasites | Macrophages infected with ≥4 parasites | ||
Resident peritoneal macrophages | No stimulation | 85 ± 3 | 248 ± 10 | 38 ± 4 |
IFN-γ (100 U) | 84 ± 4 | 238 ± 22 | 35 ± 4 | |
IFN-γ (100 U) + MCP-1 (1 ng) | 84 ± 3 | 249 ± 6 | 32 ± 2 | |
MCP-1 recruited macrophages | No stimulation | 81 ± 3 | 232 ± 18 | 34 ± 3 |
IFN-γ (100 U) | 84 ± 3 | 235 ± 16 | 33 ± 5 | |
MCP-1 (1 ng) | 87 ± 2 | 219 ± 8 | 30 ± 3 | |
IFN-γ (100 U) + MCP-1 (1 ng) | 72 ± 4* | 159 ± 15** | 19 ± 2** |
A total of 100 macrophages were counted in various fields by fluorescent microscopy.
That is, the mean number counted on three different coverslips. *, Significantly different compared to either unstimulated, IFN-γ-stimulated, or MCP-1-stimulated samples (P < 0.05); **, significantly different compared to either unstimulated, IFN-γ-stimulated, or MCP-1-stimulated samples (P < 0.01).
Macrophages were infected with wild-type L. major parasites.