FIG. 8.

Induction of TNF-α mRNA by MM6 cells incubated with muropeptides 6 to 9. Mono Mac 6 cells were stimulated with medium alone (control), medium containing LPS (10 and 0.1 ng/ml), or DAP-containing muropeptides 6 to 9 (100 μM each) for 1.5 h before RNA was isolated for RT-PCR analysis of TNF-α message (40 PCR cycles). mRNA of the 18S ribosomal gene amplified under the same conditions was used as an internal control. Asterisks indicate significant differences from untreated (control) cells (P < 0.05).