FIG. 2.
(A) Uptake of radiolabeled l-lactate by N. gonorrhoeae. Bacteria were incubated in 5 μM [l-14C]lactate, and uptake was measured over time. The mutant lacking NGO1449 (GP900) does not assimilate [l-14C]lactate, while the parental and complemented strains rapidly take up this carbon source; the strains in all panels are shown in the key. Growth kinetics of the wild-type strain (F62Smr), the lctP mutant (GP900), and the complemented mutant (GP922) in defined media supplemented with glucose (panel B, 28 mM) or lactate (panel C, 28 mM) as the sole carbon sources are shown. The lctP mutant failed to grow with lactate as the sole carbon source but showed no replication defect when grown with glucose.