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. 2006 Dec 18;75(3):1280–1290. doi: 10.1128/IAI.01525-06

FIG. 2.

FIG. 2.

Expression of PRRs upstream of IFN-β production in uninfected oviduct epithelial cells. Total cell RNA was extracted from Bm1.11 cells, reverse transcribed, and amplified with the TLR and CARD primers listed in Table 1. (A) The oviduct epithelial cells have mRNA for TLR3 but lack mRNA for TLR4 and TLR7 to TLR9. (B) The oviduct epithelial cells have mRNA for the CARD domain proteins RIG-I and MDA-5. The RT reactions were performed in both the presence (+) and the absence (−) of the avian myeloblastosis virus reverse transcriptase (indicated as RT in the figure). Specific PCR products are indicated by asterisks. Size markers in base pairs are indicated on the left margin.