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Gene expression levels in blood, MNCs, and PMNs. Determination, by qRT-PCR, of genes encoding proinflammatory cytokines and anti-inflammatory cytokine IL-10 after a 1-h coincubation with C. albicans. The expression levels are normalized to leukocytes incubated without C. albicans. The results are pooled triplicate incubations from a single donor.
