TABLE 1.
Predicted meningococcal OM proteins identified in this studya
| Category or protein nameb | NCBI locus tag (NMB no.) | No. of peptides in:
|
Molecular mass (kDa)c | pIc | Predicted function (COGS)d | ||
|---|---|---|---|---|---|---|---|
| OM | OMV | LPS− OM | |||||
| PorA outer membrane protein | 1429 | 16 | 26 | 10 | 42.1 | 9.3 | Cell wall/membrane biogenesis |
| PorB outer membrane proteine | 2039 | 14 | 20 | 13 | 35.7 | 7.8 | Cell wall/membrane biogenesis |
| Opc proteinf | 1053 | 9 | 16 | 7 | 30.0 | 10.0 | Not in COGS |
| PilQ protein | 1812 | 8 | 10 | 4 | 82.4 | 9.8 | Intracellular trafficking and secretion |
| Macrophage infectivity potentiator | 1567 | 5 | 2 | 9 | 28.9 | 5.8 | Posttranslational modification, protein turnover, and chaperones |
| Rmp proteine | 0382 | 4 | 7 | 2 | 26.2 | 7.6 | Cell wall/membrane biogenesis |
| Cell binding factor, putative | 0345 | 4 | 3 | 3 | 31.4 | 9.5 | Posttranslational modification, protein turnover, and chaperones |
| Outer membrane protein Omp85 | 0182 | 3 | 10 | 5 | 88.4 | 8.9 | Cell wall/membrane biogenesis |
| Multidrug efflux pump channel protein | 1714 | 2 | 7 | 1 | 50.4 | 8.6 | Cell wall/membrane biogenesis |
| Outer membrane protein P1, putative | 0088 | 2 | 3 | 1 | 50.5 | 9.6 | Lipid transport and metabolism |
| Adhesion and penetration protein | 1985 | 2 | 2 | 1 | 159.9 | 9.4 | Cell wall/membrane biogenesis |
| Organic solvent tolerance protein, putative | 0280 | 1 | 3 | 0 | 84.2 | 8.9 | Cell wall/membrane biogenesis |
| Outer membrane protein NspAg | 0663 | 1 | 2 | 0 | 18.4 | 9.8 | Cell wall/membrane biogenesis |
| VacJ-related protein | 1961 | 1 | 0 | 0 | 29.5 | 4.8 | Cell wall/membrane biogenesis |
| TbpBh | 0460 | 1 | 0 | 0 | 77.4 | 6.0 | Not in COGS |
| TbpAh | 0461 | 0 | 3 | 1 | 102.0 | 9.6 | Inorganic ion transport and metabolism |
| Maltose phosphorylase | 0390 | 0 | 2 | 2 | 85.4 | 5.5 | Carbohydrate transport and metabolism |
| TonB-dependent receptor | 1497 | 0 | 2 | 0 | 104.2 | 9.6 | Inorganic ion transport and metabolism |
| Conserved hypothetical protein | 2134 | 0 | 2 | 0 | 69.8 | 8.6 | Cell wall/membrane biogenesis |
| Phospholipase A1 putative | 0464 | 0 | 1 | 0 | 42.9 | 8.9 | Cell wall/membrane biogenesis |
| Conserved hypothetical protein | 1333 | 0 | 1 | 0 | 65.7 | 10.3 | Cell cycle control, mitosis, and meiosis |
| Lactoferrin binding protein A | 1540 | 0 | 1 | 0 | 105.6 | 9.6 | Inorganic ion transport and metabolism |
| Serine type peptidase | 1998 | 0 | 1 | 0 | 157.6 | 9.2 | Cell wall/membrane biogenesis |
| Conserved hypothetical protein | 2050 | 0 | 1 | 0 | 66.6 | 5.5 | Unknown |
| Cardiolipin synthetase family protein | 1434 | 0 | 1 | 0 | 57.3 | 9.3 | Lipid transport and metabolism |
| Phosphoribosylformylglycinamidine synthase | 1996 | 0 | 0 | 1 | 143.8 | 5.4 | Nucleotide transport and metabolism |
Proteins predicted by the PSORTb algorithm (located at http://psort.org/) to have an outer membrane location which were detected in the OM preparations.
The protein name is the name in the annotation of the MC58 genome located at http://www.tigr.org/ unless indicated otherwise.
The theoretical molecular mass and pI values were calculated using the ProteinLynx Global Server Package (version 2.05).
Predicted functional categories were derived from the Clusters of Orthologous Groups of proteins (COGS) database (located at http://www.ncbi.nlm.nih.gov/sutils/). Similar results were obtained in an analysis of a second independent preparation of each fraction.
The name in the annotation of the MC58 genome located at http://www.tigr.org/ differs from the previously published name used in the text. See reference 20.
The name in the annotation of the MC58 genome located at http://www.tigr.org/ differs from the previously published name used in the text. See reference 29.
See reference 24.
The name in the annotation of the MC58 genome located at http://www.tigr.org/ differs from the previously published name used in the text. See reference 42.