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Construction of suicide vector pYA3467 and generation of RpoS+ strain in serotype Typhi Ty2. (A) Construction of suicide vector pYA3467 (9,618 bp) used to generate the serotype Typhi Ty2 RpoS+ strain. (B) Crossover between suicide vector pYA3467 and chromosome of serotype Typhi Ty2. (C) Catalase test for determination of RpoS activity.
