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. 2006 Dec 18;75(3):1382–1392. doi: 10.1128/IAI.00888-06

FIG. 2.

FIG. 2.

Construction of suicide vector pYA3735 and generation of serotype Typhi with regulated rpoS expression (ΔPrpoS183::TTaraCPBADrpoS). (A) Construction of suicide vector pYA3735 (7,281 bp) used to generate serotype Typhi ΔPrpoS183::TTaraCPBADrpoS. (B) Crossover between suicide vector pYA3735 and chromosome of serotype Typhi. (C) Agarose gel (0.8%), a PCR product from chromosomal DNA from wild-type (770-bp) and ΔPrpoS183::TTaraCPBADrpoS (2,100-bp) mutants. Lanes: 1, χ3744 serotype Typhi ISP1820 RpoS+; 2, χ3769 serotype Typhi Ty2 RpoS−; 3, χ8438 serotype Typhi Ty2 RpoS+; 4, χ9066 serotype Typhi ISP1820 RpoS+ ΔPrpoS183::TTaraCPBADrpoS; 5, χ9067 serotype Typhi Ty2 RpoS− ΔPrpoS183:: TTaraCPBADrpoS; 6, χ9068 serotype Typhi Ty2 RpoS+ ΔPrpoS183::TTaraCPBADrpoS. (D) Catalase test for detection of ΔPrpoS183::TTaraCPBADrpoS without and with growth in the presence of 0.2% Ara.