TABLE 3.
Synthesis of Vi polysaccharide (μg of Vi/500 mg of total serotype Typhi protein) of serotype Typhi strains grown on LB agar (pH 7) supplemented with different amounts of NaCl at 37°Ca
| NaCl concn (mM) | Vi antigen concn (μg/mg) for indicated strain
|
||||
|---|---|---|---|---|---|
| χ3769 RopS− | χ3744 RpoS+ | χ8438 RpoS+ | χ9060 RpoS− | χ9061 RpoS− | |
| 0 | 14.01 ± 0.01 | 3.74 ± 0.03 | 0.31 ± 0.4 | 10.59 ± 0.04 | 9.61 ± 0.06 |
| 150 | 15.48 ± 0.03 | 11.08 ± 0.05 | 8.14 ± 0.09 | 14.01 ± 0.05 | 13.52 ± 0.03 |
| 300 | 14.01 ± 0.06 | 5.70 ± 0.04 | 5.20 ± 0.07 | 14.01 ± 0.03 | 13.03 ± 0.08 |
| 400 | 10.10 ± 0.06 | 0.80 ± 0.2 | 0.80 ± 0.3 | 14.01 ± 0.07 | 13.03 ± 0.05 |
a
The rocket lengths were transformed to the Vi antigen concentrations by the following equation: Vi (μg) = 4.8916 × (rocket length [cm]) − 5.0643. The standard deviations correspond to two independent experiments.