FIG. 4.

TLR expression in DCs. (A) Cell surface expression of TLR4 on untreated DCs (left) and control monocyte cultures (right). Shaded histograms indicate cells stained with isotype controls; open histograms represent cells stained with anti-TLR4 monoclonal antibody. (B) RT-PCR analysis of TLR2 and TLR4 mRNAs in untreated DCs. The bottom panel shows results for the “no RT” controls. The experiment for which the results are shown was one of two showing identical results. CTL, cytotoxic T lymphocyte.