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. 2006 Dec 11;75(3):1349–1358. doi: 10.1128/IAI.01467-06

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FIG. 2. — Immunoblot analysis of purified recombinant PyMSP-1 and PyMSP-8 antigens. (A) Immunoblots of 12% SDS-polyacrylamide gels containing (lane 1) rPyMSP-1₄₂ (0.1 μg), (lane 2) GST-PyMSP-1₁₉ (0.1 μg), and (lane 3) GST (0.1 μg) were probed with mAb302 that recognizes epitopes associated with the EGF-like domains of PyMSP-1 or with normal mouse serum (NMS). (B) Immunoblots of 12% SDS-polyacrylamide gels containing (lane 1) rPyMSP-8 (0.1 μg), (lane 2) GST-PyMSP-8C (0.1 μg), and (lane 3) GST (0.1 μg) were probed with polyclonal rabbit serum raised against refolded rPyMSP-8 or normal rabbit serum (NRS). Molecular mass markers are indicated.