FIG. 4.

Design, production, and analysis of a chimeric PyMSP-1 and PyMSP-8 vaccine. (A) Cartoon of the chimeric PyMSP-1/8 expression construct with sequences encoding the double EGF-like domains of PyMSP-1₁₉ (stippled box) followed by a five-glycine spacer (filled box) and the full-length PyMSP-8 including the C-terminal EGF-like domains (open and hatched boxes). Cysteine residues are indicated by vertical lines. (B and C) Coomassie-blue-stained SDS-polyacrylamide gel (12%) of purified rPyMSP1/8 (3 μg/lane) under reducing (R) or nonreducing (NR) conditions (B) and the corresponding immunoblot analysis of nonreduced rPyMSP-1/8 probed with (lane 1) PyMSP-1₁₉-specific mAB302, (lane 2) normal mouse serum, (lane 3) rabbit anti-rPyMSP-8 serum, or (lane 4) normal rabbit serum (C). Molecular weight markers (MW) (in thousands) are indicated.