. 2006 Dec 11;75(3):1310–1317. doi: 10.1128/IAI.00996-06

TABLE 1.

Oligonucleotide primers used for amplification

S. agalactiae reference strain (reference[s])	Gene in reference strain	Name of primer	Sequence (5′-3′)	Amplified gene(s)
176H4A (25)	fbsA	fbsA86	ATCAAGTCCTGTATCTGCTAT	fbsA
		fbsA555rc	TTCATTGCGTCTCAAACCG
		fbsA282	CAACTTATAGGGAAAAATCCAC	fbsA and truncated fbsA
		fbsA405rc	AGTTAACATCGGTCTATTAGC
NEM316 (12, 13)	gbs0850	fbsB349	ATGAAGCGATTGTGAATAGAA	fbsB^a
		fbsB1926rc	TCGCCTTGATAGCAGTGTC
		fbsB143	TCGGTCATAAAATAGCGTATGG	fbsB^a
		fbsB1710rc	AAGAATTCAACGGTCGGCTTCGT
4:74 (15)	fgag	fgag	ACGATGCTATTTCGGCGTAT	fbsB^a and all fgag variants
		fgagrc	AAGAATTCAACGGTCGGCTTCGT
090R (28)	lmb	lmb130	GTTGTGAGTTTAGTAATGATAGCGA	lmb
		lmb970rc	TATGTCTTGTTCCGCTTG
BM110 (17, 20)	gbs2018	ST-17S	ATACAAATTCTGCTGACTACCG	gbs2018-ST-17^b
		ST-17AS	TTAAATCCTTCCTGACCATTCC
NEM316 (12, 17)	dltR	dltRS	TTGACAGGTCTCTATGATTTAGTC	dltR
		dltRAS	GTCTGGTTCTCAGCCTAATTC

fbsB is fbsB gene sensu stricto as described by Gutekunst et al. (13), also known as fgagV2 (15) or fbsB2 (5).

gbs2018-ST-17 is an allelic form of the gbs2018 gene, specific for the ST-17 clone (17).