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. 2006 Dec 18;75(3):1335–1342. doi: 10.1128/IAI.01203-06

FIG. 1.

FIG. 1.

Effects of recombinant coactivating cytokines on IFN-γ production by F. tularensis LVS-stimulated HMC in vitro. (A) Hepatic mononuclear cells from B6 mice were cultured with F. tularensis LVS at the indicated MOI in the presence or absence of recombinant IL-12 (50 pg/ml), and IFN-γ production was measured 24 h later by ELISA. Cells incubated in the absence of bacteria produced 68 pg of IFN-γ per ml. (B) HMC activated in vitro at an MOI of 66 were stained with APC-conjugated anti-NK1.1 and PE-conjugated anti-IFN-γ. (C) Comparison of the effects of rIL-12 and rIL-18 in F. tularensis LVS-challenged HMC cultures (MOI, 30). (D) Synergistic effects of rIL-12 and rIL-18 on IFN-γ production by HMC challenged at an MOI of 30.