FIG. 5.
Effects of rIL-12 on IFN-γ responses to F. tularensis LVS in vivo. (A) Wild-type B6 mice were injected i.p. with the indicated doses of F. tularensis LVS, and their sera were collected 16 h later for analysis of IFN-γ or IL-12p70. (B and C) At two different F. tularensis LVS doses, one group of mice was injected with 0.5 μg of rIL-12 just prior to bacterial challenge. Either 5 × 104 CFU was given to 4 mice per group (B) or 4 × 105 CFU was given to 8 mice per group (C). In both cases, the result for each animal is shown, with horizontal lines representing the mean of each group. The groups receiving F. tularensis LVS plus rIL-12 (open circles) were significantly different from the groups receiving the same dose of F. tularensis LVS alone (P < 0.05).