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IFN-γ and IL-12 production by HMC challenged in vitro with F. tularensis subsp. tularensis clinical isolates KU49 and KU54. Cell culture supernatant fluids were collected after 24 h, and IFN-γ (A), IL-12p70 (B), and IL-12p40 (C) concentrations were measured by ELISA. Cells incubated in the absence of bacteria produced 110 pg of IFN-γ per ml.
