TABLE 1.
IL-12p70 and IFN-γ responsesa to F. tularensis in IL-12p35-deficient mice
| Genotype | Concn (ng/ml) of the following cytokine in serumb:
|
% IFN-γ+ cellsc | |
|---|---|---|---|
| IL-12p70 | IFN-γ | ||
| IL-12p35+/+ | 2.9 ± 0.70 | 57 ± 1.4 | 6.9 ± 2.4 |
| IL-12p35−/− | 0.028 ± 0.022 | 0.26 ± 0.07 | 0.2 ± 0.1 |
a
Values are means ± standard deviations for each group of mice. Four animals were used in each group.
b
Mice were injected i.p. with 106 CFU of F. tularensis LVS, and sera were collected 16 h later. The concentrations of IL-12p70 and IFN-γ were determined by ELISA. The levels of IL-12p70 and IFN-γ in normal mouse serum were below the limit of detection (25 pg/ml).
c
HMC were also isolated from these infected mice and analyzed by flow cytometry (see Fig. 6).