FIG. 3.

Dexamethasone (Dex) does not inhibit LPS-induced LAP mRNA expression, nor does LPS or dexamethasone alter TLR-4 mRNA expression. (A) Cultured TECs were treated with (+) or without (−) dexamethasone (10⁻⁶ M) for 24 h prior to the addition of LPS (100 ng/ml) for 16 h. Data represent the pooled mean (± SEM) ratios of LAP mRNA expression relative to GAPDH mRNA from triplicate primary cultures from three separate animals (*, P < 0.05 versus untreated cells). (B) Cells were treated as described for panel A, but data represent the pooled mean (± SEM) ratios of TLR-4 mRNA expression relative to GAPDH mRNA from triplicate primary cultures from three separate animals.