FIG. 5.
Substance P, acetylcholine, norepinephrine, and epinephrine do not alter TAP mRNA expression in cultured TECs. (A) TECs were exposed to substance P (SP) (10−6, 10−8, 10−10, or 10−12 M) or vehicle for 30 min prior to the addition of LPS (100 ng/ml) for 16 h. (B) Acetylcholine (Ach) (10−5, 10−6, or 10−7 M) or vehicle was added to TECs for 3 h prior to the addition of LPS (100 ng/ml) for 16 h. TECs were treated with norepinephrine (NE) (10−5, 10−6, 10−7, or 10−8 M) (C) or epinephrine (Epi) (10−4, 10−6, 10−8, or 10−10 M) (D) for 30 min prior to the addition of LPS (100 ng/ml) for 16 h. Data represent the mean ± SEM ratios of TAP mRNA expression relative to GAPDH mRNA from triplicate primary cultures. Representative data from three separate animals are shown. +, with; −, without.