TABLE 2.
Predicted group B Streptococcus surface-associated proteins (n = 48) detected exclusively and in greater abundance under highly invasive conditions (td of 1.8 h and 12% DO2) than under poorly invasive conditions (td of 1.8 h and 0% DO2)a
| Function or description and TIGR locus | Putative identification | Gene | Average Sequest Xcorr value | Ratio of identified peptides |
|---|---|---|---|---|
| Cell envelope formation | ||||
| SAG0159 | Penicillin-binding protein 1B, putative | 3.2 | ||
| SAG0287 | Penicillin-binding protein 2X | pbpX | 3.4 | |
| SAG0757 | Protein of unknown function/lipoprotein, putative | 3.9 | ||
| SAG0765 | Penicillin-binding protein 2b | 3.4 | ||
| SAG1419 | Lipoprotein, putative | 2.9 | ||
| SAG2066 | Penicillin-binding protein 2A | pbp2A | 3.5 | |
| SAG0298 | Penicillin-binding protein 1A | pbp1A | 3.4 | 3.0 |
| SAG0405 | Protein of unknown function/lipoprotein, putative | 3.1 | 7.0 | |
| SAG1173 | CpsC protein | cpsC | 2.3 | 4.0 |
| SAG1787 | DltD protein | dltD | 3.6 | 6.0 |
| Cellular processes | ||||
| SAG0017 | PcsB protein | pscB | 4.1 | |
| SAG0016 | Cell division protein FtsH | ftsH | 3.4 | 2.8 |
| SAG0478 | Cell division protein FtsA | ftsA | 3.5 | 4.0 |
| SAG0479 | Cell division protein FtsZ | ftsZ | 3.4 | 5.0 |
| Energy metabolism | ||||
| SAG0859 | ATP synthase Fo, beta subunit | atpF | 3.4 | |
| SAG0940 | 6-Phosphofructokinase | pfk | 2.7 | |
| SAG0941 | Pyruvate kinase | pyk | 3.5 | 4.5 |
| Fatty acid and phospholipid metabolism | ||||
| SAG0342 | Enoyl-coenzyme A hydratase/isomerase family protein | 3.1 | ||
| Conserved hypothetical proteins | ||||
| SAG0304 | 3.4 | |||
| SAG0455 | 3.0 | |||
| SAG1361 | 4.3 | |||
| SAG1774 | 3.8 | |||
| SAG2068 | 2.9 | |||
| SAG1139 | 3.2 | 3.0 | ||
| SAG1613 | 2.8 | 8.0 | ||
| Regulatory functions | ||||
| SAG0319 | Serine/threonine protein kinase | 3.7 | 2.3 | |
| SAG0624 | Septation ring formation regulator ExrA, putative | 3.5 | 19 | |
| Protein fate | ||||
| SAG1828 | ATP-dependent Clp protease, ATP-binding subunit | 2.7 | ||
| SAG1914 | Membrane-associated zinc metalloprotease, putative | 4.0 | ||
| SAG2174 | Serine protease | 3.5 | ||
| Signal transduction | ||||
| SAG0394 | Sensor histidine kinase | 2.9 | ||
| Transcription | ||||
| SAG0777 | ATP-dependent RNA helicase, DEAD/DEAH box family | 3.3 | ||
| Transport and binding | ||||
| SAG0514 | Cation-transporting ATPase, E1-E2 family | 3.7 | ||
| SAG1108 | Spermidine/putrescine ABC transporter, spermidine/putrescine-binding protein | potD | 3.4 | |
| SAG1257 | Cation-transporting ATPase, E1-E2 family | 3.3 | ||
| SAG1262 | Cation-transporting ATPase, E1-E2 family | 2.6 | ||
| SAG1337 | ABC transporter, ATP-binding/permease protein | 4.2 | ||
| SAG1359 | Permease, putative | 2.7 | ||
| SAG1732 | Glycerol uptake facilitator protein, putative | 4.0 | ||
| SAG1007 | Iron-compound ABC transporter, iron-compound-binding protein | 3.0 | 2.3 | |
| SAG1393 | Iron compound ABC transporter, substrate-binding protein | 3.5 | 3.5 | |
| SAG1466 | Glutamine ABC transporter, glutamine-binding protein/permease | glnP | 3.0 | 5.5 |
| SAG1796 | Amino acid ABC transporter, permease protein | 3.4 | 7.0 | |
| Unknown function | ||||
| SAG0019 | Aminotransferase, class I | 3.2 | ||
| SAG0306 | KH domain protein | 2.9 | ||
| SAG1186 | Metallo-beta-lactamase superfamily protein | 2.5 | ||
| SAG1381 | Sulfatase | 3.1 | 7.0 | |
| SAG1628 | LemA protein | lemA | 2.9 | 5.0 |
a
Surface-associated proteins (n = 3) whose genes were also revealed by genomics are shown in boldface.