TABLE 6.
Inhibition of group B Streptococcus type V adherence to ME-180 cells by antiserum to selected surface-associated proteins and the regulation of expression of these proteins
| TIGR locus | Ratio chip | Average Xcorr value | Annotation | Gene | Inhibition of adherence ± SD (%) | Prediction assay, regulationa |
|---|---|---|---|---|---|---|
| SAG1350 | NAb | 2.9 | Surface antigen-related protein | 96.3 ± 1.4 | P, high growth rate | |
| SAG0971 | 1.9 | NA | Lipoprotein of unknown function/lipoprotein, putative | 93.5 ± 1.8 | G, high growth rate | |
| SAG0765 | 1.7 | 3.4 | Penicillin-binding protein 2b | 90.4 ± 2.0 | G, high growth rate; P, high growth rate and oxygen | |
| SAG0823 | 2.0 | NA | Glyceraldehyde-3-phosphate dehydrogenase, NADP dependent | gapN | 89.6 ± 1.0 | G, high growth rate |
| SAG1007 | 2.0 | 3.0 | Iron compound ABC transporter, iron compound-binding protein | 85.5 ± 4.2 | G, oxygen; P, high growth rate and oxygen | |
| SAG0017 | NA | 4.1 | PcsB protein | pscB | 66.5 ± 8.0 | P, high growth rate and oxygen |
| SAG1466 | 2.3 | 3.0 | Glutamine ABC transporter, glutamine-binding protein/permease protein | glnP | 57.3 ± 9.6 | G, high growth rate and oxygen; P, high growth rate and oxygen |
| SAG1938 | NA | NA | Positive control, LRA family, Lmb (59%) | 92.0 ± 1.0 |
a
G, genomics; P, proteomics; high growth rate, td = 1.8 h; oxygen, 12% DO2.
b
NA, not applicable.