FIG. 2.

The presence of NK1.1⁺ CD11c⁺ cells in the spleen. (A to C) Splenocytes from a naive C57BL/6 mouse were stained with anti-NK1.1-PE and anti-CD11c-ALPC in the presence or absence of 10 mM EDTA (A), anti-NK1.1-PE and normal hamster IgG-ALPC (isotype control for anti-CD11c-ALPC) (B), and normal mouse IgG2a-PE and anti-CD11c-ALPC (C). Plots were gated on live splenocytes, and the numbers in each quadrant indicate the percentages of cells in each quadrant. (D to F) The percentage of CD11c⁺ cells from sorted NK1.1⁺ cells. Enriched NK1.1⁺ cells (by MACS) were stained with anti-NK1.1-PE and then sorted on the NK1.1⁺ population. The purity of sorted cells is presented in panel D. (E) The sorted NK1.1⁺ cells were then cytospun and stained for Ly49G2 (red) and CD11c (green). Arrows in the plots indicate cells stained both green and red, while symbols *, #, and & denote cells stained green or red or without any staining, respectively. (F) From stained slides, the percentages of Ly49G2⁺, CD11c⁺, and Ly49G2⁺ CD11c⁺ cells were determined. Twelve random photographs were taken from each of four slides, and the results of the four slides were averaged and presented. The presented data are representative of two independent experiments. Error bars represent the standard deviations.