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. 2006 Dec 11;75(3):1167–1176. doi: 10.1128/IAI.01026-06

FIG. 6.

FIG. 6.

NK1.1+ CD11+ cells are present in naive spleen, LN, blood, and bone marrow, and their amount increases after infection. (A and B) Splenocytes, blood, LN, and bone marrow cells and thymocytes from naive C57BL/6 mice were stained for NK1.1 and CD11c. (A) The representative flow plots display NK1.1 staining on the y axis and CD11c staining on the x axis. The plots are gated on total live cells. The numbers in the quadrants indicate the percentages of cells in each quadrant. (B) Percentages of NK1.1+ CD11+ cells of total NK1.1+ cells from each indicated organ are presented. The results are the averages from four mice from one of two independent experiments. Error bars represent the standard deviations. (C to F) Splenocytes, blood, LN, and bone marrow cells from naive and 72 hpi C57BL/6 mice were stained for NK1.1 and CD11c. (C) The representative flow plots display NK1.1 staining on the y axis and CD11c staining on the x axis as described above. (D) Percentages of NK1.1+ CD11+ cells of total NK1.1+ cells from each indicated organ are presented. (E) The total number of NK1.1+ CD11c+ cells in spleen is presented. (F) The total number of NK1.1+ CD11c+ cells in LN and femur is presented. The P value between naive mice and 72 hpi mice is <0.01 for each indicated organ. The results are the averages from three mice per group from one of two independent experiments. Error bars represent the standard deviations.