FIG. 3.
TLR2-mediated endogenous IL-5 and eotaxin production are involved in eosinophil recruitment and activation induced by BCG. IL-5 (A) or eotaxin (B) production were evaluated in the pleural wash from TLR2+/+ and TLR2−/− mice stimulated in vivo by BCG (5 × 106 bacilli/cavity) or vehicle within 24 h by enzyme-linked immunosorbent assay (Duo set kit; R&D Systems, Minneapolis, MN). The effect of neutralization of eotaxin or CCR3 on pleural eosinophil accumulation (C) and lipid body formation (D) induced by BCG infection was also analyzed. Treatments (neutralizing antibodies at 10 μg/animal; R&D Systems) were administered intraperitoneally 30 min before intrapleural injection of BCG (5 × 106 bacilli/cavity), and eosinophil analysis was performed within 24 h. Each bar represents the mean ± the SEM from at least four animals. Differences between control and infected groups are indicated by asterisks (P < 0.05); an “#” symbol represents differences between treated and untreated BCG-infected groups or between wild-type and BCG-deficient mice.