Fig. 6.

The effects of wiskostatin on the kinetics of CFTR internalization. A, biotinylated CFTR in lysates of BHK cells treated with vehicle or wiskostatin (100 μM) for 2 h at 37° C followed by biotinylation at 0° C. Cells were rewarmed to 37° C for indicated times and the labeled CFTR remaining at the cell surface was stripped by MESNA. Note that the surface CFTR signal was much less for the wiskostatin-treated cells as compared to vehicle control (see also Fig. 1). B, CFTR signals in control or wiskostatin-treated cells were normalized to the respective surface CFTR signals and plotted as a function of time. The data points represent means ± S.E. of 2–7 experiments.