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. 2007 Mar 28;2(3):e330. doi: 10.1371/journal.pone.0000330

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Agaugué et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a, b) MLR were conducted as described in figure 4 with control LPS-matured DC (filled bars), LPS-matured DC pre-treated with SB203580 (SB) (grey bars), 24 h LVP-treated DC matured with LPS (opened bars), DC pre-treated with SB before LVP incubation and LPS treatment (hatched bars). Secretion of cytokine was normalized to 100% for control LPS-treated DC. IFNγ (a) and IL-5 and IL-13 (b) were measured by ELISA in supernatants of co-culture. Mean±sd are shown. Secretions are in the range of 260–2600 pg/ml for IFNγ, 20–80 pg/ml for IL-5 and 150–370 pg/ml for IL-13. (c, d) MLR were conducted as described in figure 4 with control LPS-matured DC (filled bars), LPS-matured DC pre-treated with PD98059 (PD) (grey bars), 24 h LVP-treated DC matured with LPS (opened bars), DC pre-treated with PD before LVP incubation and LPS treatment (hatched bars). Secretion of cytokine was normalized to 100% for control LPS-treated DC. IFNγ (c) and IL-5 and IL-13 (d) were measured by ELISA in supernatants of co-culture.