TABLE 4.
dspE and hrpN promoter activities of wild-type E. chrysanthemi 3937 (Ech3937) and iaaM deletion mutant (Ech138) grown in MM
| Time of culture (h) | Activity for gene and promotera:
|
|||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ech3937(PdspE)
|
Ech138(PdspE)
|
Ech3937(PhrpN)
|
Ech138(PhrpN)
|
Ech3937(PhrpL)
|
Ech138(PhrpL)
|
|||||||||||||
| Total | GFP+ mean | GFP+% | Total | GFP+ mean | GFP+% | Total | GFP+ mean | GFP+ % | Total | GFP+ mean | GFP+% | Total | GFP+ mean | GFP+% | Total | GFP+ mean | GFP+% | |
| 6 | 70 ± 7 | 131 ± 1 | 52 ± 5 | 4 ± 0 | 43 ± 7 | 1 ± 0 | 16 ± 4 | 143 ± 2 | 9 ± 3 | 3 ± 0 | 69 ± 23 | 1 ± 0 | 11 ± 0 | 20 ± 1 | 37 ± 1 | 7 ± 0 | 16 ± 0 | 16 ± 0 |
| 12 | 107 ± 2 | 148 ± 2 | 72 ± 1 | 7 ± 0 | 48 ± 1 | 8 ± 1 | 46 ± 6 | 126 ± 4 | 35 ± 6 | 12 ± 0 | 106 ± 5 | 9 ± 1 | 14 ± 1 | 23 ± 1 | 46 ± 2 | 9 ± 0 | 18 ± 0 | 27 ± 1 |
a
The promoter activities were compared after 6 and 12 h of culture in MM. GFP intensity was determined on gated populations of bacterial cells by flow cytometry. Values (mean fluorescence intensity) are representative of two experiments. Three biological replicates were used in this experiment. “Total” represents the average GFP fluorescence intensity of total bacterial cells, “GFP+ mean” represents the average GFP fluorescence intensity of GFP-expressing bacterial cells, and “GFP+%” represents GFP-expressing bacterial cells as a percentage of the total bacterial cells.