TABLE 5.
pelD, dspE, and hrpN promoter activities of wild-type E. chrysanthemi 3937 (Ech3937) and iaaM deletion mutant (Ech138) grown in African violet cv. Gauguin (Saintpaulia ionantha)
| Strain with gene promoter | Activity at 24 ha
|
||
|---|---|---|---|
| Total | GFP+ mean | GFP+% | |
| Ech3937(PpelD) | 68 ± 6 | 171 ± 14 | 35 ± 0 |
| Ech138(PpelD) | 23 ± 7 | 142 ± 17 | 12 ± 5 |
| Ech3937(PdspE) | 87 ± 42 | 184 ± 27 | 42 ± 17 |
| Ech138(PdspE) | 15 ± 4 | 111 ± 2 | 9 ± 2 |
| Ech3937(PhrpN) | 65 ± 16 | 239 ± 5 | 26 ± 6 |
| Ech138(PhrpN) | 14 ± 14 | 182 ± 14 | 6 ± 7 |
a
The promoter activities were compared after 24 h of inoculation. GFP intensity was determined on gated populations of bacterial cells by flow cytometry. “Total” represents the average GFP fluorescence intensity of total bacterial cells, “GFP+ mean” represents the average GFP fluorescence intensity of GFP-expressing bacterial cells, and “GFP+%” represents GFP-expressing bacterial cells as a percentage of the total bacterial cells. Values (mean fluorescence intensity) are representative of two experiments. Three biological replicates were used in this experiment.