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Detection of E. coli O157:H7 by RAMS cultures from cattle. Three 5-month-old Holstein steers were penned separately. RAMS samples were cultured for E. coli O157 as indicated. Animal no. 135 was carrying a natural bovine E. coli O157 and was not challenged with a bacterial dose. Animal no. 711 received a single oral dose and animal no. 407 received a single rectal dose of 1010 CFU E. coli O157:H7 ATCC 43894 at day zero. E+, culture positive by enrichment culture only.
