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Agarose gels showing amplicons from RT-PCRs performed using cultures amended with toluene (T) or benzoate (B). Amplification was carried out using specific bssA-2 primers (panel A) and Eub-1 (16S rRNA gene) primers (panel B) listed in Table 2. Expected amplicon lengths were ∼300 bp for all reactions. Control reactions shown: “T-RT” and “B-RT,” controls without reverse transcriptase; +, culture DNA; −, no sample.
