TABLE 2.
PCR primers used for amplifying specific genes in the toluene-degrading consortium
| Primera | Sequence (5′-3′) | Target gene |
|---|---|---|
| Eub1-621f | TGAAACCATTGAGCTTGAGG | Eub-1 (16S rRNA gene) |
| Eub1-912r | CCCGTCAATTCCTTTGAGTT | Eub-1 (16S rRNA gene) |
| bssA1-416f | CAGAACACAAAGTATGCCC | bssA-1 |
| bssA1-749r | TGGAGTTCCAGAGGTCGATT | bssA-1 |
| bssA2-413f | TACCTGCAGAGCAAGTACGC | bssA-2 |
| bssA2-713r | ACTTCAGCTTATCGGCGTTC | bssA-2 |
a
The annealing temperature for all primer sets was 59°C. Estimated sequence positions are listed in primer names.