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Placement of the Flavobacterium 16S rRNA sequence into a phylogenetic tree using ARB, revealing affinity with F. hibernum. An approximately 1.3-kb region of the 16S rRNA gene was amplified using forward primer 63f and reverse primer 1387r and sequenced. The phylogenetic tree was constructed by neighbor-joining analysis of partial 16S rRNA sequences according to the ARB manual (27). The bar represents 10% sequence divergence.
