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. 2006 Dec 22;73(4):1089–1100. doi: 10.1128/AEM.01577-06

FIG. 5.

FIG. 5.

Serial deletions of HU promoter. (A) The N-terminal primer D1 to D7 with a KpnI site and C-terminal primer gfpSphIR with an SphI site were used to amplify the deletion derivatives. Left dotted arrows represent the N-terminal primers (D1 to D7). The right dotted arrow represents the C-terminal primer gfpSphIR. The solid box represents gfpmut3. P.C., the positive control (SCH41); N.C., the negative control (SCH03). (B) Quantitative analysis of GFP production of the deletion series in F. hibernum strain W22. (C) Quantitative analysis of GFP production of the deletion series in F. johnsoniae.