Alignment of putative Flavobacterium RBS regions. The 30-bp nucleotide sequences upstream of the start codon were chosen for comparison. The sources of the selected genes are as follows: a, F. hibernum strain W22; b, F. johnsoniae; c, F. columnare; d, P. heparinus; and e, F. psychrophilum. The putative consensus RBSs are indicated as boldface and capitalized. These were defined as nucleotides that are present at any given position in more than 50% of the sequences. The AT contents within 30 bp and 16 bp upstream of the start codon are given as percentages.