TABLE 2.

Primers used for amplifying, sequencing, and phylotyping

Gene	Primer designationa	Sequence of primer (5′-3′)	PCR conditions (°C)b	Reference
gdhA	GdhAF	GATGGATGACGGCCGCATCG	63	This study
	GdhAR	TGAACGCCGCCGTCCGCAG	63	This study
adk	AdkF	TCTGTTGGGCGCACCCGGC	62	This study
	AdkR	CCCAGCCGGAGTAGTAGTCC	62	This study
	AdkNF	CGCCGGCAAAGGTACGCAAG	63	This study
	AdkNR	CGGGCGGGTCTGGTTCTCG	63	This study
gyrB	GyrBF	AGGGCTTCGTCGAGTACATCAA	62	This study
	GyrBR	GTTCCGCCGAGGCTCCACG	62	This study
	GyrBNF	GTGGAACGACGGCTTCAACGA	63	This study
	GyrBNR	GCGCGAGAACTGGTACTGCC	63	This study
gapA	GapAF	ATGACCATCAAGATCGGCAT	55	This study
	GapAR	GGGCCATTTCCAGCACCT	55	This study
ppsA	PpsAF	CTGTACAACGACCGCGCTAT	55	This study
	PpsAR	GTTGGTCAGGCCCATCTCTT	55	This study
	PpsANF	GGGCGTGATGTTCACGAT	57	This study
	PpsANR	CCAGCATGGGGTTCTCTTC	57	This study
fliC	Rsol_fliCF	GAACGCCAACGGTGCGAACT	63	55
	Rsol_fliCR	GGCGGCCTTCAGGGAGGTC	63	55
hrpB	RShrpBF	TGCCATGCTGGGAAACATCT	64	47
	RShrpBR	GGGGGCTTCGTTGAACTGC	64	47
egl	EglF	AAATCCAGATATCGAATTGCCAA	57	This study
	EglR	GCGTGCCGTACCAGTTCTG	57	This study
	Endo-Fc	ATGCATGCCGCTGGTCGCCGC	70	47
	Endo-Rc	GCGTTGCCCGGCACGAACACC	70	47
	Nmult:21:1F	CGTTGATGAGGCGCGCAATTT	58	7
	Nmult:21:2F	AAGTTATGGACGGTGGAAGTC	58	7
	Nmult:23:AF	ATTACSAGAGCAATCGAAAGATT	58	7
	Nmult:22:InF	ATTGCCAAGACGAGAGAAG TA	58	7
	Nmult:22:RR	TCGCTTGACCCTATAACGAGTA	58	7
	759	GTCGCCGTCAACTCACTTTCC	58	7
	760	GTCGCCGTCAGCAATGCGGAATCG	58	7

^aF, forward primer; R, reverse primer; N, internal primer (nested).

^bReady-for-use annealing temperatures. All PCRs were preceded by a 5-min denaturation step at 96°C, followed by a final extension step of 5 min at 72°C.

^cPrimer set used as internal primers.