FIG. 1.

Schematic of chemotaxis assay using two 96-well plates. B. burgdorferi cells in motility buffer plus methylcellulose were placed in wells of a 96-well plate. Holes were formed in the corresponding bottoms of wells of another 96-well plate with a heated metal cylinder. These 96-well plates were taped together face to face, with the plate containing the cells lying face up. Capillary tubes filled with attractant in motility buffer plus methylcellulose were plugged at one end with silicone grease and placed through the holes in the top 96-well plate. The ends of the capillary tubes dipped into the cell suspension in the corresponding wells of the other 96-well plate. The sandwiched plates were incubated such that the attractant-filled capillary tubes were horizontal but still remained inserted in the cell suspension.