FIG. 7.

cheA2 inactivation and complementation. (a) Ten micrograms of protein from lysates of wild-type, cheA2, and cheA2+ cells were electrophoresed on sodium dodecyl sulfate-polyacrylamide gels, blotted onto nitrocellulose, and reacted with E. coli CheA and B. burgdorferi DnaK antibodies. (b) Southern blot analysis of chromosomal or plasmid DNA from cheA2+, wild-type, and cheA2 mutant cells (see the text). Southern blot analysis was carried out with a digoxigenin-labeled DNA probe complementary to a fragment of cheA2 deleted in the mutant (Table 1). The chromosomal and plasmid DNAs migrated at rates represented by the vertical axis.