TABLE 1.

Primers used to amplify genetic elements

Fragment	Primer name	Primer sequencea
flgB promoter	pflgB1-forward	5′-CTGCAG TAATACCCGAGCTTCAAG-3′
	pflgB2-reverse	5′-CATATGACCTCCCTCATTTAAAATTGC-3′
cheA2	FPA2-forward	5′-CATATGATTAAAGAGGAGAAATTAACTATG-3′
	RPA2-reverse	5′-GCATGCTCAGCTAATTAAGCTTGGCTG-3′
T7 terminator	pFT7-forward	5′-GCATGCGCTAACAAAGCCCGAAAGGAAGCT-3′
	pRT7-reverse	5′-AAGCTTTGCAGATCCGGATATAGTTCCTCC-3′
cheA2 probe	DFA2-forward	5′-AAAAGACAATCCTATGGCTAC-3′
	DFA2-reverse	5′-TAACTCTCTCTTCAACTGTTTC-3′

^aEngineered restriction sites are underlined.