Figure 1. Comparison of Heat Shock Sensitivity for the WT Strain, and the pka1 and pkr1 Mutants.

(A) Cells (10⁵) were subjected to heat shock treatment at 50 °C for the indicated time and spotted on YPD plates. The plates were incubated for 3 d at 30 °C.

(B) Enumeration of colony forming units (CFUs) for the strains after heat shock. The data are expressed as the percent of the starting number of cells recovered after heat shock and represent the mean values ± standard deviation (SD) from three independent experiments.