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. 2007 Mar 16;3(3):e42. doi: 10.1371/journal.ppat.0030042

Figure 2. Suppression of Capsule Formation by Secretion Inhibitors.

Figure 2

(A) Sensitivity of the WT strain H99 and the pka1 and pkr1 mutants to trafficking inhibitors. Photographs of cells on medium containing the ferrous iron chelator BPS, and the chelator plus monensin, are included to demonstrate growth of the strains under low iron conditions similar to those used in the broth cultures (B) to measure capsule size.

(B) Suppression of capsule formation in both the WT strain and the pkr1 mutant after treatment with trafficking inhibitors at the indicated concentrations. Cells were cultured in LIM at 30 °C and examined at 72 h after addition of the inhibitors indicated. Chemicals and concentrations are indicated at the top and the strains are noted on the left of the photographs. Bar = 10 μM.

(C and D) Sixty cells from the WT (H99) strain (C) and the pkr1 mutant (D) were measured to determine cell diameter and capsule radius with and without treatment with the trafficking inhibitors (as shown in [B]). The capsule sizes of the treated cells were statistically different (p <0.001) from the sizes for the untreated cells in all cases. Each bar represents the average of 60 measurements.